Ukusebenza Okuphezulu Kwe-Liquid Chromatography/HPLC
I-High Performance Liquid Chromatography/HPLC yaziwa nangokuthi "i-high-pressure liquid chromatography", "high-speed liquid chromatography", "high-resolution liquid chromatography", "modern column chromatography", njll. Ukusebenza okuphezulu kwe-chromatography yoketshezi kuyigatsha elibalulekile i-chromatography.Isebenzisa uketshezi njengesigaba esihambayo futhi isebenzisa isistimu yokufaka umfutho ophezulu ukumpompa izinyibilikisi ezingazodwa ezine-polarity ezihlukene noma izincibilikisi ezixubekile, izibhafa nezinye izigaba ezihambayo ezinezilinganiso ezihlukene zibe yisigaba esimile.Ngemva kokuba izingxenye zekholomu ye-chromatographic zihlukene, zingena kumtshina ukuze zitholwe futhi zihlaziywe isampula.Le ndlela isibe ubuchwepheshe obubalulekile bokuhlukanisa nokuhlaziya emkhakheni wamakhemikhali, imithi, imboni, i-agronomy, ukuhlolwa kwempahla kanye nokuhlolwa kwezomthetho.
Izici zokusebenza okuphezulu kwe-chromatography ye-liquid:
① Ukucindezela okuphezulu: Isigaba esihambayo siwuketshezi.Uma igeleza kukholomu ye-chromatographic, ihlangabezana nokuphikiswa okukhulu.Ukuze udlule ngokushesha kukholamu ye-chromatographic, uketshezi lwenkampani yenethiwekhi kufanele lucindezelwe.
②Ukusebenza kahle okuphezulu: ukusebenza kahle kokuhlukaniswa okuphezulu.Isigaba esimile kanye nesigaba esihambayo singakhethwa ukuze kuzuzwe umphumela wokuhlukanisa ongcono kakhulu, ophakeme izikhathi eziningi kunokusebenza kahle kokuhlukaniswa kwemibhoshongo yezimboni kanye ne-gas chromatography.
③Ukuzwela okuphezulu: Umtshina we-UV ungafinyelela ku-0.01ng.
④Uhla olubanzi lwezinhlelo zokusebenza: Ngaphezu kuka-70% wezinto eziphilayo zingahlaziywa ngokusebenza okuphezulu kwe-chromatography yoketshezi.
⑤ Isivinini sokuhlaziya okusheshayo nezinga lokugeleza koketshezi lenkampani yenethiwekhi esheshayo: ngokushesha kakhulu kune-chromatography yoketshezi yakudala
Ngaphezu kwalokho, i-chromatography ye-liquid esebenza kahle kakhulu namakholomu e-chromatography angasetshenziswa ngokuphindaphindiwe, amasampula awawonakali, futhi kulula ukuwabuyisela.Nokho, nazo zinezinkinga.Uma kuqhathaniswa ne-chromatography yegesi, ngayinye inezinzuzo zayo futhi iyaphelelisana.
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Isikhathi sokuthumela: Nov-23-2023
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